Major category: Biological Sciences Minor category: Medical Sciences Title: Molecular phylogeny of a newfound hantavirus in the Japanese shrew mole (Urotrichus talpoides) Running title: Hantavirus in the Japanese Shrew Mole Authors:

نویسندگان

  • Satoshi D. Asakawa
  • Mitsuhiko Kang
  • Hae Ji
  • Satoru Arai
  • Satoshi D. Ohdachi
  • Mitsuhiko Asakawa
  • Hae Ji Kang
  • Gabor Mocz
  • Jiro Arikawa
  • Nobuhiko Okabe
  • Richard Yanagihara
  • John A. Burns
چکیده

Recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. Apart from challenging the conventional view that rodents are the principal reservoir hosts, the newly identified soricid-borne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. In analyzing RNA extracts from lung tissues of the Japanese shrew mole (Urotrichus talpoides), captured in Japan between February and April 2008, a novel hantavirus genome, designated Asama virus (ASAV), was detected by RT-PCR. Pair-wise alignment and comparison of the S-, Mand L-segment nucleotide and amino acid sequences indicated that ASAV was genetically more similar to hantaviruses harbored by soricine shrews than rodents. However, the predicted secondary structure of the ASAV nucleocapsid protein was similar to that of rodentand shrew-borne hantaviruses, exhibiting the same coiled-coil helix at the amino terminus. Phylogenetic analyses, using the maximumlikelihood method and other algorithms, consistently placed ASAV with recently identified soricine shrew-borne hantaviruses, suggesting a possible host-switching event in the distant past. The discovery of a hantavirus in the Japanese shrew mole enlarges our concepts about the evolutionary history of hantaviruses.

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Molecular phylogeny of a newfound hantavirus in the Japanese shrew mole (Urotrichus talpoides).

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تاریخ انتشار 2017